GMO Research
Abstract: Roundup® is extensively used for weed control worldwide. Residues of this compound may lead to side effects of the male reproductive system. However, the toxic effects and mechanisms of Roundup® of male germ cells remain unclear. We aimed to investigate the apoptosis-inducing effects of Roundup® on mouse male germ cells and explore the role …
Abstract: Roundup® is extensively used for weed control worldwide. Residues of this compound may lead to side effects of the male reproductive system. However, the toxic effects and mechanisms of Roundup® of male germ cells remain unclear. We aimed to investigate the apoptosis-inducing effects of Roundup® on mouse male germ cells and explore the role …
Abstract: Objective: To investigate the protective effect of different doses of tea polyphenol (TP) on the mouse sertoli cells against oxidative damage and apoptosis induced by glyphosate (GLY). Methods: The oxidative damage model of primarily cultured mouse sertoli cells was established through GLY (90mg/L) incubation for 24h. Then the cells were treated with different doses …
Abstract: Objective: To investigate the effect of different doses of glyphosate on apoptosis and expressions of androgen-binding protein (ABP) and vimentin mRNA in mouse Sertoli cells. Methods: Primarily cultured mouse Sertoli cells incubated with different doses of glyphosate (60, 90, 120, 150 and 180 mg/L) for 24 h. The growth and morphological alterations in the …
Abstract: Glyphosate-based herbicides are extensively used for weed control all over the world. Therefore, it is important to investigate the putative toxic effects of these formulations which include not only glyphosate itself but also surfactants that may also be toxic. 3T3-L1 fibroblasts are a useful tool to study adipocyte differentiation, this cell line can be …
Abstract: Glufosinate ammonium structurally resembles glutamate and blocks glutamine synthetase. Glufosinate was recently found to be dysmorphogenic in mammals in vitro. The present study examined the cell death induced specifically by glufosinate in the neuroepithelium of mouse embryos. Electron micrograph revealed characteristic chromatin condensation and segregation, extracellular apoptotic bodies, and cell fragments phagocytosed in macrophages …
Abstract: Glyphosate, a common herbicide, is not toxic under normal exposure circumstances. However, this chemical, when combined with a surfactant, is cytotoxic. In this study, the mechanism of the additive effect of glyphosate and TN-20, a common surfactant in glyphosate herbicides, was investigated. After exposure of rat H9c2 cells to glyphosate and TN-20 mixtures, following …
Abstract: In this study, it has been investigated that the effects of glyphosate, which is a herbicide within organophosphate and unselective widely used in agriculture on enzyme activity of carbonic anhydrase, production of reactive oxygen species, cell apoptosis and body morphology during the embryonic development of zebrafish. To this end, it has been treated embryo …
Abstract: The goal of the present study was to elucidate the in vitro cytotoxicity of Roundup and to reveal the possible related mechanisms in L-02 hepatocytes. By detecting reactive oxygen species (ROS) production, glutathione (GSH)/superoxide dismutase (SOD) levels, mitochondrial permeability transition pore (PTP) open rate, apoptosis-inducing factor (AIF) release, intracellular Ca2+ concentration, and alanine aminotransferease (ALT)/aspartate aminotransferase (AST) …
Abstract: Occupational exposures to pesticides may increase parental risk of infertility and adverse pregnancy outcomes such as spontaneous abortion, preterm delivery, and congenital anomalies. Less is known about residential use of pesticides and the risks they pose to reproduction and development. In the present study we evaluate environmentally relevant, low-dose exposures to agrochemicals and lawn-care …
Abstract: Agricultural genetically modified (GM) plants are essentially plants which contain pesticides, because they were designed to tolerate or produce pesticides. In 2011, GM crops reached 160 million hectares, with 59 % of herbicide tolerance (mainly Roundup) mostly in soybean, maize, canola, cotton, 15 % of insecticide producing varieties and 26% combining both traits (James …
Abstract: A deregulation of programmed cell death mechanisms in human epidermis leads to skin pathologies. We previously showed that glyphosate, an extensively used herbicide, provoked cytotoxic effects on cultured human keratinocytes, affecting their antioxidant capacities and impairing morphological and functional cell characteristics. The aim of the present study, carried out on the human epidermal cell …
Abstract: In this study, cell death detected by DNA fragmentation labeling and phosphatidylserine (PS) localization was investigated in the honey bee (Apis mellifera L.) midgut, salivary glands and ovaries after treating larvae with different pesticides offered via an artificial diet. To do this, honey bee larvae reared in an incubator were exposed to one of …
Abstract: We demonstrated that glyphosate possesses tumor promoting potential in mouse skin carcinogenesis and SOD 1, calcyclin (S100A6), and calgranulin B (S100A9) have been associated with this potential, although the mechanism is unclear. We aimed to clarify whether imbalance in between [Ca2+]i levels and oxidative stress is associated with glyphosate-induced proliferation in human keratinocytes HaCaT …
Abstract: The study of combined effects of pesticides represents a challenge for toxicology. In the case of the new growing generation of genetically modified (GM) plants with stacked traits, glyphosate‐based herbicides (like Roundup) residues are present in the Roundup‐tolerant edible plants (especially corns) and mixed with modified Bt insecticidal toxins that are produced by the GM plants …
Abstract: Herbicides have been recognized as the main environmental factor associated with human neurodegenerative disorders such as Parkinson’s disease(PD). Previous studies indicated that the exposure to glyphosate, a widely used herbicide, is possibly linked to Parkinsonism, however the underlying mechanism remains unclear. We investigated the neurotoxic effects of glyphosate in differentiated PC12 cells and discovered …
Abstract: Glyphosate is a widely used broad spectrum herbicide, reported to induce various toxic effects in non-target species, but its carcinogenic potential is still unknown. Here we showed the carcinogenic effects of glyphosate using 2-stage mouse skin carcinogenesis model and proteomic analysis. Carcinogenicity study revealed that glyphosate has tumor promoting activity. Proteomic analysis using 2-dimensional …
Abstract: BACKGROUND. Worldwide used pesticides containing different adjuvants like Roundup formulations, which are glyphosate-based herbicides, can provoke some in vivo toxicity and in human cells. These pesticides are commonly found in the environment, surface waters and as food residues of Roundup tolerant genetically modified plants. In order to know their effects on cells from liver, …
Abstract: To further investigate the possible relationships between agricultural pesticide exposure and the increased risk of non-Hodgkin’s Lymphoma among farm workers in the north central United States, we performed G-banded chromosome analyses of peripheral blood from workers classified according to primary types of pesticide exposure: herbicides (n = 20), insecticides (n = 18), fumigants (n …
Abstract: 目的: 探讨农达(41%草甘膦)对人L-02肝细胞产生的损伤作用及其可能的机制。 方法: 体外试验(in vitro test)以L-02肝细胞为受试细胞,通过MTT法检测不同浓度的农达对L-02肝细胞存活率的影响,选择细胞存活率为20%-80%的浓度进行后续实验。设置5个农达处理组,60mg/L、90mg/L、120mg/L、150mg/L、180mg/L和1个阴性对照组(PBS),农达处理细胞时间为24h。L-02肝细胞的形态学改变采用姬母萨染色法在光镜下观察结合透射电镜观察;农达对L-02肝细胞膜通透性影响和细胞毒性作用采用谷草转氨酶(AST)、谷丙转氨酶(ALT)活性水平结合台盼蓝拒染法来评价;农达诱导L-02肝细胞氧化损伤程度选用超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽(GSH)含量等指标评价;用线粒体跨膜电位(△Ψm)检测细胞线粒体损伤;用DNA条带(DNA-Ladder)检测肝细胞DNA损伤;以AnnexinV-FITC/PI复染法测定细胞发生凋亡和坏死的情况;Western Blotting检测对照组与90mg/L组细胞色素C(Cyt C)和凋亡诱导因子(AIF)表达水平。 结果: 1.在60～180mg/L的处理浓度范围内,能明显引起L-02肝细胞存活率的降低(P＜0.05),处理浓度和细胞存活率之间存在负相关(r=-0.974)。 2.在光镜下观察姬母萨染色L-02肝细胞爬片,观察到各农达处理组L-02肝细胞皱缩或肿大,形态由瓦片状收缩为圆形,细胞间隙扩大,细胞破裂,染色质浓缩,贴壁细胞密度和数量减少。电镜下观察,可发现处理组细胞出现表面微绒毛消失,细胞膜结构不完整,细胞核碎裂或肿胀,线粒体空泡样变等细胞凋亡或坏死的表现。 3.农达处理L-02肝细胞,其活细胞台盼兰蓝染比例升高(P＜0.05);细胞培养上清液中ALT活性增加(P＜0.05);从90mg/L组开始AST活性增加(P＜0.05);农达导致处理组细胞内MDA的含量增加,SOD活性减弱,GSH含量减少(P＜0.05);农达导致120～180mg/L组细胞Na~+-K~+ ATP酶活性降低(P＜0.05)。 4.农达处理能诱导细胞DNA链断裂,处理浓度在150mg/L、180mg/L,DNA受损程度严重;90mg/L组Cyt C和AIF表达水平高于对照组(P＜0.05);90mg/L组开始用农达处理的L-02细胞线粒体膜电位明显降低(P＜0.05);农达能明显诱导处理组肝L-02细胞发生凋亡和坏死(P＜0.05),随着处理农度的增高,细胞凋亡和坏死的比例增高,但是在180mg/L组凋亡比例下降。 结论: 农达在60mg/L～180mg/L范围内,能引起L-02肝细胞存活率下降,细胞膜通透性增加,抑制细胞离子转运,诱发DNA损伤,线粒体膜电位降低,Cyt C、AIF等凋亡因子泄漏,使细胞产生凋亡和坏死,对肝细胞具有明显的损伤作用;其损伤的作用机制可能与农达导致肝细胞氧化损伤、线粒体崩溃等途径有关。 Keywords: glyphosate, human L-02 hepatocytes, in vitro, AST, ALT, SOD, apoptosis, Roundup, malondialdehyde, glutathione Citation: Fei, W., 2008. Roundup (41% glyphosate) research on human L-02 liver cell injury. South University.
Abstract: Among the molecules to which the human skin is exposed, glyphosate is used as an herbicide. Glyphosate has been shown to induce in vitro cutaneous cytotoxic effects, concomitant with oxidative disorders. In this following study, we focused on dynamic events of the loss of HaCaT cell integrity appearing after a glyphosate treatment. In these …
