Search Results for: Microbiota
Safety evaluation of neo transgenic pigs by studying changes in gut microbiota using high-throughput sequencing technology
Abstract: The neo (neomycin phosphotransferase) gene is widely used as a selection marker in the production of genetically engineered animals and plants. Recent attention has been focused on safety concerns regarding neo transgene expression. In this study, neo transgenic and non-transgenic piglets were randomly assigned into Group A and Group B to evaluate effects of …
Glyphosate based-herbicide exposure affects gut microbiota, anxiety and depression-like behaviors in mice
Abstract: Recently, a number of studies have demonstrated the profound relationship between gut microbiota (GM) alterations and behavioral changes. Glyphosate-based herbicides (GBH) have been shown to induce behavioral impairments, and it is possible that they mediate the effects through an altered GM. In this study, we investigated the toxic effects of GBH on GM and …
Residual effect of transgenic soybean in soil microbiota
Abstract: Today, Brazil is the second major soybean (Glycine max (L.) producer of the world; however, few studies on the effects of transgenesis in the soil microbiota are completed. This study aims at assessing the residual effect of a transgenic soybean with tolerance to the imidazolinone herbicide group on the soil microbiota. The parameters examined …
Analysis of caecal microbiota in rats fed with genetically modified rice by real-time quantitative PCR
Abstract: The effect of genetically modified rice (GMR) on bacterial communities in caecal content was analyzed in a 90-d feeding rat model. A total of 12 groups of rats, which included male and female, were fed with the basal diets containing 30%, 50%, 70% GMR (B(1), B(2), B(3)) or 30%, 50%, 70% non-GMR (D(1), D(2), …
The effect of glyphosate on potential pathogens and beneficial members of poultry microbiota in vitro
Abstract: The use of glyphosate modifies the environment which stresses the living microorganisms. The aim of the present study was to determine the real impact of glyphosate on potential pathogens and beneficial members of poultry microbiota in vitro. The presented results evidence that the highly pathogenic bacteria as Salmonella Entritidis, Salmonella Gallinarum, Salmonella Typhimurium, Clostridium …
Effects of Roundup (glyphosate) on gut microorganisms of farm animals
Abstract: Glyphosate, is a broad-spectrum, nonselective, systemic herbicide, existing in the form of the acid itself or formulated as a salt. Roundup consists of the glyphosate isopropylamine (IPA) salt, water and a surfactant, often a polyethoxylated tallowamine, POEA. Glyphosate inhibits 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) in the shikimate pathway, which leads to formation of aromatic amino acids. …
Sex-dependent impact of Roundup on the rat gut microbiome
Abstract: A growing body of research suggests that dysbiosis of the gut microbiota induced by environmental pollutants, such as pesticides, could have a role in the development of metabolic disorders. We have examined the long-term effects of 3 doses of the Roundup(R) herbicide (made of glyphosate and formulants) on the gut microbiota in male and female …
The Ramazzini Institute 13-week pilot study on glyphosate and Roundup administered at human-equivalent dose to Sprague Dawley rats: Effects on the microbiome
Abstract: Background Glyphosate-based herbicides (GBHs) are broad-spectrum herbicides that act on the shikimate pathway in bacteria, fungi, and plants. The possible effects of GBHs on human health are the subject of an intense public debate for both its potential carcinogenic and non-carcinogenic effects, including its effects on microbiome. The present pilot study examines whether exposure …
Neutralization of the antimicrobial effect of glyphosate by humic acid in vitro
Abstract: In the present study, the neutralization ability of the antimicrobial effect of glyphosate by different humic acids was investigated. The minimal inhibitory concentrations of glyphosate for different bacteria such as Bacillus badius, Bifidobacterium adolescentis, Escherichia coli, E. coli 1917 strain Nissle, Enterococcus faecalis, Enterococcus faecium, Salmonella enteritidis and Salmonella typhimurium were determined in the presence …
Insecticidal toxin from Bacillus thuringiensis is released from roots of transgenic Bt corn in vitro and in situ
Abstract: The insecticidal toxin encoded by the cry1Ab gene from Bacillus thuringiensis was released in root exudates from transgenic Bt corn during 40 days of growth in soil amended to 0, 3, 6, 9, or 12% (v/v) with montmorillonite or kaolinite in a plant growth room and from plants grown to maturity in the field. …
Microbiological attributes in a latosol in glyphosate application under water deficit conditions
Abstract: The objective of this study was to evaluate the effects of glyphosate on soil microbial activity under appropriate and water deficit conditions. The factors consisted of four treatments with different Roundup Ready® soybean cultivars, two herbicidal treatments [glyphosate (1.080 and 1.800 g a.e. ha-1) + control] and two soil moisture treatments (appropriate and water …
Glyphosate suppresses the antagonistic effect of Enterococcus spp. on Clostridium botulinum
Abstract: During the last 10-15 years, an increase of Clostridium botulinum associated diseases in cattle has been observed in Germany. The reason for this development is currently unknown. The normal intestinal microflora is a critical factor in preventing intestinal colonisation by C. botulinum as shown in the mouse model of infant botulism. Numerous bacteria in …
Oral application of charcoal and humic acids to dairy cows influences Clostridium botulinum blood serum antibody level and glyphosate excretion in urine
Abstract: The present study was initiated to investigate the influence of oral application of charcoal, sauerkraut juice and humic acids on specific gastrointestinal microbiota and selected enzymes, electrolytes, and substrates in the blood of dairy cows fed GMO feeds containing glyphosate. A total of 380 Schleswig Holstein cows suffering from symptoms of chronic botulism were …
