Search Results for: Ovine

Abstract: To assess the likelihood that the bla gene present in a transgenic maize line may transfer from plant material to the microflora associated with animal feeds, we have examined the survival of free DNA in maize silage effluent, ovine rumen fluid and ovine saliva. Plasmid DNA that had previously been exposed to freshly sampled …

Abstract: Glyphosate is a widely used broad-spectrum herbicide that has expanded its applications on plant varieties that are genetically modified to tolerate glyphosate treatment. The aim of this study was to determine the frequency of micronuclei (MNi) in bovine peripheral lymphocytes after exposure to glyphosate in vitro. The cytokinesis block micronucleus assay (CBMN) for estimation of …

Abstract: The persistence of plant-derived recombinant DNA in sheep and pigs fed genetically modified (Roundup Ready) canola was assessed by PCR and Southern hybridization analysis of DNA extracted from digesta, gastrointestinal (GI) tract tissues, and visceral organs. Sheep (n = 11) and pigs (n = 36) were fed to slaughter on diets containing 6.5 or 15% …

Text: The galactopoietic potency of bovine somatotrophin (bST; also known as growth hormone, bGH) has been known for over 50 years, but it is only since the 1980s that production of bST by recombinant DNA techniques has raised the prospect of its large scale use in dairying. Subcutaneous or intramuscular injections of approximately 30mg/day can …

Text: In November 1998, an expert panel appointed by the Canadian VMA (CVMA) at the request of Health Canada, but working independently, found “a number of legitimate animal welfare concerns associated with the use of rBST” (recombinant bovine somatotropin). These included an increased risk of clinical mastitis and lameness, and a reduction in the lifespan …

Abstract: This manuscript presents the results of a review of the effects of recombinant bovine somatotropin (rBST) on milk production, milk composition, dry matter intake, and body condition score that was carried out by an expert panel established by the Canadian Veterinary Medical Association (CVMA). The panel was established by the CVMA in response to …

Abstract: This manuscript presents the results of a review of the effects of recombinant bovine somatotropin (rBST) on dairy cattle health, reproductive performance, and culling, that was carried out by an expert panel established by the Canadian Veterinary Medical Association (CVMA). The panel was established by the CVMA in response to a request from Health …

Abstract: A technical herbicide containing isopropyl amine salt of glyphosate was tested for induction of chromosome aberrations (CA) and sister chromatid exchanges (SCE) in cultured bovine peripheral lymphocytes. Cultures were exposed to a glyphosate formulation at concentrations ranging from 28 to 1120 micromol/l without and with metabolic activation. No clastogenic effect of the herbicide was …

Abstract: Contrary to assertions in the editorial and Special Communication, administration of bST does significantly change milk composition, mainly reflecting tissue utilization to make extra milk, such as an increase in long-chain fatty acids. One importantexception is the increase in insulinlike growth factor I (IGF-I) to levels found usually in the first 2 weeks of …

Keywords: recombinant bovine growth hormone, rBGH, recombinant bovine somatotropin, rBST Citation: Kronfeld, D.S., 1993. Concerns about bovine somatotropin. Journal of the American Veterinary Medical Association, 203(2), p.190.

Keywords: recombinant bovine growth hormone, rBGH, recombinant bovine somatotropin, rBST Citation: Kronfeld, D.S., 1988. Biologic and economic risks associated with use of bovine somatotropins. Journal of the American Veterinary Medical Association, 192(12), p.1693.

Abstract: Six lactating, non-pregnant Jersey cows were given subcutaneous injections of recombinantly derived bovine growth hormone for 7 d. Milk yield was increased by 4.5 kg/d on d 7, compared with the average yield of 10.7 +/- 0.4 kg/d (mean +/- s.e.m.) for the 7 d preceding treatment. Concentrations of insulin-like growth factor I (IGF-I) …

Citation: Challacombe, D.N., Wheeler, E.E., Collier, R., Clemmons, D., Donovan, S. and Wilkinson, J.I., 1994. Safety of milk from cows treated with bovine somatotrophin. The Lancet, 344(8925), pp.815-817.

Abstract: Usage of the herbicide glyphosate on core crops in the USA has increased exponentially over the past two decades, in step with the exponential increase in autoimmune diseases including autism, multiple sclerosis, inflammatory bowel disease, type 1 diabetes, coeliac disease, neuromyelitis optica and many others. In this paper we explain how glyphosate, acting as …

Abstract: Firstly, he does not point out that, although scientists claim this technology will help to feed the world’s burgeoning population, food production is not the problem. There are enough natural resources for us all to be fed: inequity of food distribution results in the starvation of millions in poorer nations. Secondly, he dismisses the …

Abstract: The polymerase chain reaction (PCR) technique was used to investigate the fate of a transgene in the rumen of sheep fed silage and maize grains from an insect-resistant maize line. A 1914-bp DNA fragment containing the entire coding region of the synthetic cryIA(b) gene was still amplifiable from rumen fluid sampled 5 h after …

Keywords: recombinant bovine growth hormone, rBGH, recombinant bovine somatotropin, rBST Citation: Kronfeld, D.S., 2001. Concerned about use of BST in dairy cattle. Journal of the American Veterinary Medical Association, 219(5), pp. 591-2.

Keywords: recombinant bovine growth hormone, rBGH, recombinant bovine somatotropin, rBST Citation: Kronfeld, D.S., 1989. BST milk safety. Journal of the American Veterinary Medical Association, 195(3), pp. 288-9.

Abstract: The Biotechnology and Food Safety Report issued jointly by the Food and Agriculture Organization (FAO) and World Health Organization (WHO), is the result of an Expert Consultation held in Rome, in October 1996. The Consultation was the latest, possibly the most significant, attempt to reach international agreement on the safety of genetically engineered (GE) …

Abstract: Recently we demonstrated that recombinant Cry1Ac protoxin from Bacillus thuringiensis is a potent systemic and mucosal immunogen. In this study we compared the adjuvant effects of Cry1Ac and cholera toxin (CT) for the hepatitis B surface antigen (HBsAg) and bovine serum albumin (BSA). The antibody responses of intestinal secretions and serum were determined by …

Abstract: The aim of this study was to trace selected nucleic acid and protein components of isogene versus Bt transgene maize within the bovine gastrointestinal tract (GIT). After feeding 22 cattle for 4 weeks with Bt176 maize, different plant genes and the recombinant protein CryIAb were quantified during digestion. Furthermore, a first initial characterization of …

Text: Yu and Rohan (1) are to be commended for their comprehensive literature review of the mitogenic and apoptotic actions of insulin-like growth factor (IGF)-I and of the epidemiologic evidence incriminating elevated levels of circulating IGF with excess risks of breast, colorectal, prostate, and other cancers. This evidence is supported by the excess risks of …

Abstract: Levels of insulin-like growth factor-1 (IGF-1) are substantially elevated and more bioactive in the milk of cows hyperstimulated with the biosynthetic bovine growth hormones rBGH, and are further increased by pasteurization. IGF-1 is absorbed from the gastrointestinal tract, as evidenced by marked growth-promoting effects even in short-term tests in mature rats, and absorption is …

Abstract: The use of biosynthetic milk hormones raises fundamental ethical, social, and economic considerations, including the continued viability of the small family dairy farm and adverse veterinary effects. The past and expanding use of synthetic bovine growth hormone manufactured by the Agricultural Chemicals Division of Elanco (Eli Lilly and Co.) in conjunction with Dow Chemical …