Search Results for: Intestine
Dynamics of the microbiocenosis of intestines in pigs at conditions of using of transgenic soya
Abstract: It has been researched the microbiocenosis of caudal area of rectum of pigs which were had the diet with genetic modified soya. It was determined the specific composition of microorganisms, which were developed on nutritious mediums after the insemination them by samples of excrements: mainly Proteus vulgaris, E.Coli, Bifidobacterium, Lactobacillus, Enterococcus and also Saphylococcus …
Histochemical and histopathological study of the intestine of the earthworm (Pheretima elongata) exposed to a field dose of the herbicide glyphosate
Abstract: Histopathological and histochemical variations in non-specific esterases of the intestinal epithelial lining of the earthworm (Pheretima elongata), exposed to a single field dose of the herbicide glyphosate, were studied on the first, second, third, and fourth week of exposure. A severe cell death was observed in the intestine during the first and second weeks …
Effects of sub-lethal exposure of rats to the herbicide glyphosate in drinking water: Glutathione transferase enzyme activities, levels of reduced glutathione and lipid peroxidation in liver, kidneys and small intestine
Abstract: Glyphosate (GLP), the active ingredient of many weed killing formulations, is a broad spectrum herbicide compound. Wistar rats were exposed during 30 or 90 days to the highest level (0.7 mg/L) of GLP allowed in water for human consumption (US EPA, 2011) and a 10-fold higher concentration (7 mg/L). The low levels of exposure …
Cry1Ac protoxin from Bacillus thuringiensis sp. kurstaki HD73 binds to surface proteins in the mouse small intestine
Abstract: Bacillus thuringiensis (Bt), considered a safe insecticide, produces insecticidal proteins named Cry during sporulation, which possess exceptional immunological properties. In this work using an immunohistochemical test we demonstrated that Cry1Ac protoxin (pCry1Ac) binds to the mucosal surface of the mouse small intestine. Ligand blot assay allowed us to detect, under denaturing conditions, six pCry1Ac-binding …
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Distribution of glyphosate in chicken organs and its reduction by humic acid supplementation
Abstract: Glyphosate (N-(phosphonomethyl) glycine) is a most popular herbicide in agricultural practices throughout the world. It is possible that glyphosate spread in the ecosystems can reach plants, animals. The present work was directed to investigate the glyphosate residue in different organs of broiler chickens using ELISA and to study the possibility of its neutralisation using …
Detection of transgenic and endogenous plant DNA in digesta and tissues of sheep and pigs fed Roundup Ready canola meal
Abstract: The persistence of plant-derived recombinant DNA in sheep and pigs fed genetically modified (Roundup Ready) canola was assessed by PCR and Southern hybridization analysis of DNA extracted from digesta, gastrointestinal (GI) tract tissues, and visceral organs. Sheep (n = 11) and pigs (n = 36) were fed to slaughter on diets containing 6.5 or 15% …
Observations on the effect of glyphosate based herbicide on ultra structure (SEM) and enzymatic activity in different regions of alimentary canal and gill of Channa punctatus (Bloch)
Abstract: Glyphosate is the isopropyl amine salt of N-(Phosphonomethyl)-glycine, a broad-spectrum nonselective herbicide, which has been extensively used to control annual and perennial weeds in agricultural, forest and aquatic systems. The ultrastructural changes in different regions of alimentary canal and gill were observed by Scanning Electron Microscopic study on a non-target aquatic teleostea fish, Channa …
Foreign (M13) DNA ingested by mice reaches peripheral leukocytes, spleen, and liver via the intestinal wall mucosa and can be covalently linked to mouse DNA
Abstract: Food-ingested foreign DNA is not completely degraded in the gastrointestinal tract of mice. Phage M13mp18 DNA as a test molecule devoid of homology to mouse DNA was pipette-fed to or added to the food supply of mice. The fate of this foreign DNA in the animals was followed by several methods. In 84 animals, …
Glyphosate on digestive enzymes activity in piava (Leporinus obtusidens)
Abstract: The effects of glyphosate, a nonselective herbicide (1.0 or 5.0mg L-1) on digestive enzymes activity (stomach and intestine) were evaluated in juveniles of piava (Leporinus obtusidens) after 90 days of exposure. The activity of acid protease, trypsin, chymotrypsin and amylase increased with the increase of glyphosate concentration. These results indicate that glyphosate affects digestive …
Organs development, gene expression and health of Atlantic salmon (Salmo salar L.) fed genetically modified soybeans compared to the near‐isogenic non‐modified parental line
Abstract: The present experiment was conducted to study the possible effects of genetically modified (GM), full‐fat soybean meal (FFSBM) from Round‐up Ready® soybeans compared to its parental, and closest near‐isogenic, non‐modified (nGM) soybean variety, added at moderate (150 g kg−1) and high (300 g kg−1) inclusion levels. The fish showed a high specific growth rate (SGR …
Evaluation of stress‐ and immune‐response biomarkers in Atlantic salmon, Salmo salar L., fed different levels of genetically modified maize (Bt maize), compared with its near‐isogenic parental line and a commercial suprex maize
Abstract: The present study was designed to evaluate if genetically modified (GM) maize (Bt maize, event MON810) compared with the near‐isogenic non‐modified (nGM) maize variety, added as a starch source at low or high inclusions, affected fish health of post‐smolt Atlantic salmon, Salmo salar L. To evaluate the health impact, selected stress‐ and immune‐response biomarkers …
Genetically modified foods and the Pusztai affair
Abstract: In his clinical review on genetically modified foods Jones implies that Pusztai had tested only the effects of potato spiked with concanavalin A (a lectin) at the Rowett Institute. The initial dissemination of this incorrect information followed by the inappropriate suspension of Pusztai and the suspicion of fraud implied by instituting an audit according …
Tissues studies of tilapias fed with transgenic soybean feedstuff
Abstract: Tilapias (Oreochromis niloticus) were fed using transgenic soybean diets and non-transgenic soybean diets. Gastric contents, intestinal contents and faeces were sampled respectively from tilapias at 1h, 4h and 8h after feeding and different tissues of tilapias were collected at 4-, 7-week and continued hunger 2-week. DNA was extracted from these samples and the distribution …
Safety testing of GM-rice expressing PHA-E lectin using a new animal test design
Abstract: The 90-day animal study is the core study for the safety assessment of genetically modified foods in the SAFOTEST project. The model compound tested in the 90-day study was a rice variety expressing the kidney bean Phaseolus vulgaris lectin agglutinin E-form (PHA-E lectin). Female Wistar rats were given a nutritionally balanced purified diet with …
Degradation of Cry1Ab protein from genetically modified maize (MON810) in relation to total dietary feed proteins in dairy cow digestion
Abstract: To investigate the relative degradation and fragmentation pattern of the recombinant Cry1Ab protein from genetically modified (GM) maize MON810 throughout the gastrointestinal tract (GIT) of dairy cows, a 25 months GM maize feeding study was conducted on 36 lactating Bavarian Fleckvieh cows allocated into two groups (18 cows per group) fed diets containing either …
Does transgenic feed cause histopathological changes in rats?
Abstract: Genetically modified (GM) crops have been commercially available for human and animal consumption since the 1990s. The safety evaluations are based on the concept of substantial equivalence, which assumes that the toxicity of a product can be investigated by assessing the toxicity of individual components of the product and not the product as a …
