Search Results for: Morphologic
Disturbance in some physiological and morphological indicators of rats consuming genetically modified soy
Abstract: Data of a pilot study of the use by rats of genetically modified soy are provided in work. It is established, the long use of hypermarket of soy negatively influences resistance of rats to physical activities, leads to dystrophic changes of internals, and change of biochemical indicators of blood, and also to mass death …
Commercial formulation containing 2,4-D affects biochemical parameters and morphological indices of silver catfish exposed for 90 days
Abstract: The objective of this study was to verify whether a commercial formulation of 2,4 dichlorophenoxyacetic acid [2,4-D dimethylamine salt (DMA)] affects the growth and biochemical parameters of silver catfish (Rhamdia quelen) after 90 days of exposure. The fish exposed to 0.5 or 2.0 mg/L of DMA presented exhibited decreased growth parameters. Glucose was reduced …
Morphological and cytological features of organism of Mice, who consumed genetically modified potato (Solanum Tuberosum L.) with gene for acyl-lipid ∆9-desaturase
Abstract: The problems of the introduction of transgenic products into the consumer market were examined. The impact of the genetically modified potatoes on the cytological and morphological parameters in mice was investigated. An increase in body mass and in hearts mass and a decrease in spleens mass, degeneration of the ovaries and changing of the …
Morphological and cytological features of organism of mice, who consumed genetically modified potato (Solanum Tuberosum L.) with gene SMAMP-2
Abstract: The article is devoted to the introduction of transgenic products into the consumer market. The impact of the genetically modified potatoes on the cytological and morphological parameters of mice was investigated. Transgenic potato plants were produced by agrobacterium-mediated transformation using genetic constructions 35S-proSmAMP with marker gene npt11 of the resistance to the kanamycin. An increase in hearts …
Morphological changes in lung and heart mast cells during long-term action of such herbicides as bipyridyl phosphate and roundup in small doses
Abstract: The mast cells of lungs and heart have the reaction on the long, during 6 months, action of small doses of herbicides of dipyridylphosphate and roundup with the increase of their number and the increase of their functional activity, that become apparent uneven rise of degranulation. It is histochemical proved that these processes make …
Morphological changes of liver, kidneys and adrenal glands of experimental animals in the case of long term feeding with Roundup tolerant genetically modified soya
Abstract: Long period of time, for 485 days, feeding the pigs with thermally treated roundup GM soya in number of 15-20% rawprotein from the whole ration cause the development of the protein dystrophy of the kidneys and liver which testifies to the reduction of the adaptive opportunities of the organs. The most expressed declines from …
Pathomorphological changes in liver of rats with use of genetically modified soya
Citation: Kolousova, N.G., Gubina-Vakulik, G.I., Ivanenko, T.A., 2011, May 12-13. Pathomorphological changes in liver of rats with use of genetically modified soya. Actual Problems of Onomorphology: Materials Science Practical conference with International Participation and 3rd Conference of the Ukrainian Division of the International Academy of Pathology, Kharkiv, Ukraine / Kharkiv National University, p.100. Keywords: GM …
Study of the physiological and morphological parameters in rats and their offspring using a diet containing soybean transgenic EPSPS CP4
Text: Проводились исследования влияния диеты, содержащей генетически модифицированную (ГМ) сою, устойчивую к гербициду раундапу (Roundup Ready, линия 40.3.2, трансген EPSPS CP4), на морфологическое состояние органов взрослых крыс и выживаемость потомства (группа «ГМ-соя»). В контрольных группах крыс к виварному корму добавляли в таком же количестве традиционную сою Arcon SJ 91-330 (группа «Трад-соя») или ничего не добавляли …
Morphological damages of a glyphosate-treated human keratinocyte cell line revealed by a micro- to nanoscale microscopic investigation
Abstract: Among the molecules to which the human skin is exposed, glyphosate is used as an herbicide. Glyphosate has been shown to induce in vitro cutaneous cytotoxic effects, concomitant with oxidative disorders. In this following study, we focused on dynamic events of the loss of HaCaT cell integrity appearing after a glyphosate treatment. In these …
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Safety assessment of Roundup ready soybean
Abstract: This article summarises data about glyphosate-tolerant (“Roundup ready”) genetically modified soybean influence on animals’ organism. The influence of herbicide glyphosate on the organism of plants and animals, which is widely used herbicide in agriculture in the most countries of the world, is considered. Each year, the use of “Roundup” herbicide increases, as weeds become …
Glyphosate and Roundup® alter morphology and behavior in zebrafish
Abstract: Glyphosate has become the most widely used herbicide in the world, due to the wide scale adoption of transgenic glyphosate resistant crops after its introduction in 1996. Glyphosate may be used alone, but it is commonly applied as an active ingredient of the herbicide Roundup®. This pesticide contains several adjuvants, which may promote an unknown …
Neonatal exposure to a glyphosate based herbicide alters the development of the rat uterus
Abstract: Glyphosate-based herbicides (GBHs) are extensively used to control weeds on both cropland and non-cropland areas. No reports are available regarding the effects of GBHs exposure on uterine development. We evaluated if neonatal exposure to a GBH affects uterine morphology, proliferation and expression of proteins that regulate uterine organogenetic differentiation in rats. Female Wistar pups …
Effect of the herbicide Avans 330 SL on the liver pathomorphology of clinically healthy carp (Cyprinus carpio L.) and carp infected by Ichthyophthirius multifiliis
Abstract: This study was conducted on fingerlings of carp. Cyprinus carpio L., that were either clinically healthy or infected with a natural invasion of the ciliate, Ichthyophthirius multifiliis. The fish were exposed for 96 h to Avans 330 SL in a concentration of 2 mg trimethylosulfonium glyphosate l-1 water in order to establish its effect …
Glyphosate induces neurotoxicity in zebrafish
Abstract: Glyphosate based herbicides (GBH) like Roundup® are used extensively in agriculture as well as in urban and rural settings as a broad spectrum herbicide. Its mechanism of action was thought to be specific only to plants and thus considered safe and non-toxic. However, mounting evidence suggests that GBHs may not be as safe as …
New effects of Roundup on amphibians: predators reduce herbicide mortality; herbicides induce antipredator morphology
Abstract: The use of pesticides is important for growing crops and protecting human health by reducing the prevalence of targeted pest species. However, less attention is given to the potential unintended effects on nontarget species, including taxonomic groups that are of current conservation concern. One issue raised in recent years is the potential for pesticides …
Roundup preliminary study on male germ cells and its mechanism of toxicity
Abstract: 研究目的： 通过GC.1小鼠精原细胞体外试验及小鼠精子畸形体内试验,探讨农达对雄性生殖细胞的毒性作用及其可能作用机制。 研究方法： GC-1小鼠精原细胞体外试验：以GC-1细胞为受试细胞,通过MTT试验筛选农达染毒浓度及NAC干预浓度。其后续试验中NAC干预浓度为101μmol／L,预处理1h,农达染毒浓度定为60、90、120、150、180mg／L,染毒时间为24h。将GC-1细胞随机分成对照组(第1组)、农达染毒组(第2-6组)、10mmol／L NAC+90 mg／L农达染毒干预组(第7组)。GC-1细胞的形态学改变,采用姬母萨染色法在光镜下观察；农达对GC-1细胞膜通透性影响和细胞毒性作用采用乳酸脱氢酶(LDH)活性水平结合台盼蓝拒染法评价；农达诱导GC-1细胞氧化损伤程度选用超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽(GSH)含量指标评价；用彗星实验检测细胞DNA损伤。 小鼠精子畸形体内试验：将100只昆明种雄性小鼠随机分为5组(每组20只),即290mg／kg、580mg／kg、1160mg／kg农达染毒组、1个阴性对照组和1个阳性对照组。各染毒组和阴性对照组采用灌胃给药,阳性对照组采用腹腔注射,连续给药5天,将动物于给药第7、28、35天分三批处死,观察睾丸、附睾重量及其脏器系数,分析精子数和精子畸形率的变化。 结果： GC-1小鼠精原细胞体外试验： 1、农达在60-180mg／L处理浓度范围内,能明显引起GC-1细胞存活率的降低(P0.05),处理浓度和细胞存活率之间存在负相关(r=-0.992,p0.01)。 2、农达在60-180mg／L处理浓度范围内,引起GC-1细胞皱缩或肿大,染色质浓染,细胞间隙扩大,空泡样变。随着农达浓度的增加变化明显,细胞密度也明显减少,细胞膜和核膜不完整或消失,染色质嗜碱性降低。加入NAC有保护作用。 3、农达在90-180mg／L处理浓度范围内,引起细胞培养上清液中LDH活性增加(p0.05),台盼蓝染色实验表明农达引起细胞膜受损率增加(p0.05),NAC能减少细胞毒性。 4、农达在60-180mg／L处理浓度范围内,能明显诱导细胞DNA的链断裂,剂量越高,DNA断裂程度越严重,彗星率越高(P0.05)；NAC+90mg／L处理组,与90mg／L处理组相比DNA损伤程度减轻(P0.05)。 5、农达在60-180mg／L处理浓度范围内,随染毒剂量增大,MDA生成增多SOD、GSH活性明显降低,与对照组比较差异有显著性(P0.05)；加入NAC后有明显保护作用(P0.05)。 小鼠精子畸形体内试验： 1、与阴性对照组比较,580mg／kg、1160mg／kg农达剂量组可使小鼠精子数目明显减少(p0.05)、精子畸形率明显增加(p0.05)。 2、与阴性对照组比较,1160mg／kg农达剂量组在染毒后早期可引起附睾重量和附睾系数下降,在染毒后晚期引起附睾和睾丸重量减轻,睾丸系数下降(p0.05)。 结论： 农达在60mg／L-180mg／L范围内,能引起GC-1细胞存活率下降,LDH活性增加,MDA生成增多、GSH和SOD活性降低以及DNA损伤,使细胞产生凋亡和坏死,对细胞具有明显的损伤作用；加入抗氧化剂NAC预处理后具有相应的拮抗作用。提示其损伤的作用机制可能与农达导致GC-1细胞氧化与抗氧化失衡、破坏细胞膜通透性,引起细胞氧化损伤和DNA损害等途径有关。 580mg／kg和1160mg／kg的农达剂量组可引起小鼠精子数目减少、精子畸形率增加,以及附睾和睾丸重量及其系数下降,提示农达对雄性小鼠具有明显的生殖毒性作用。 Keywords: Roundup, Toxic Effects, Male Germ Cells, Mouse Sperm Abnormality, In Vitro Test, Morphological Changes, Cytotoxicity, Survival Rate, Cell Survival, Chromatin Staining, Cell Gap Expansion, Vacuolization, DNA Strand Breakage, Sperm Abnormality, Sperm Count Citation: …
